Biacore units use Surface Plasmon Resonance (SPR), enabling investigators to detect and monitor biomolecular binding events in real-time. This allows the user to gain a better understanding of biochemical mechanisms associated with protein-ligand interactions. The Penn Dental Medicine Biacore Facility Core features two Biacore units – the Biacore X and the Biacore 3000. The Biacore X is a manually operated unit with two flow cells, and the Biacore 3000 is a fully automated unit with four flow cells and multiple capabilities.
SPR is a valuable research tool to assay components by defining their interactions, providing quantitative information on:
A special unit installed into the Biacore 3000 is an SPR-MALDI interface block. The block is designed to recover molecules that bind to a protein fixed to the sensor surface in the small volumes that are suitable for mass spectrometry. Bound component(s) are eluted into the micro-recovery module and analyzed by mass spectrometry, which identifies the bound component(s). This has been used to:
Dr. Gary Cohen, Director
216 Levy Building