The laboratory of Dr. Koo focuses on understanding the relationship between biofilms and oral infectious diseases and seeking novel therapeutic strategies to control pathogenic biofilms, including those associated with dental caries.
Biofilms are structured communities of microbial cells that are attached to a surface and enmeshed in a self-produced three-dimensional (3D) matrix of extracellular polymeric substances (EPS). The matrix provides an essential scaffold for the initial assembly and further development of biofilms. It promotes microbial adhesion, cohesion and protection as well as hindering diffusion. Importantly, the matrix also creates spatial and microenvironmental heterogeneities in biofilms, modulating the growth and survival of pathogens locally. The matrix is considered a key factor for the existence of the biofilm lifestyle and full expression of virulence by several bacterial pathogens.
Dr. Koo’s research is particularly interested in elucidating three major questions:
(1) How the extracellular matrix assembles dynamically in 3D. In particular, we are interested in understanding the structural organization of EPS, and how they modulate cell adhesion-cohesion, the 3D matrix-scaffold and mechanical stability of biofilms over time.
(2) How the matrix modulates the microenvironmental heterogeneity within biofilms. Here, we focus on spatio-temporal characterization of local pH and oxygen levels, microbial organization/positioning and gene expression in situ, and how these properties influence the virulence of biofilms as a whole.
(3) How to disrupt the EPS production and target the pathogens embedded in the matrix. We are interested in finding new strategies to prevent biofilm initiation or disrupt existing biofilms using naturally occurring molecules, as well as using in silico methods. We are also employing novel (nano/bio)technologies to create anti-biofilm materials and target the biofilm microenvironments.
To accomplish these goals, our lab uses a combination of molecular, biochemical, imaging and biophysical techniques, which include enzymatic, confocal fluorescence microscopy, AFM/rheometry, transcriptomic-proteomic and bioengineering approaches. Several in vitro and in vivo models to study the assembly of mixed-species biofilms are available in our laboratory. We also use these biofilm models to evaluate the effectiveness of novel anti-biofilm approaches.
Dr. Koo is also the Founding Director of the Center for Innovation & Precision Dentistry, a collaboration between Penn Dental Medicine and Penn’s School of Engineering & Applied Sciences.
Honors / Credentials
- IADR William H. Bowen Research in Dental Caries Award, 2018
- NIH Adjunct Researcher (Visiting Scientist), National Institute of Dental and Craniofacial Research (NIDCR), National Institutes of Health (NIH), Bethesda, MD, 2011-2012
- Associate Professor (tenured) of Dentistry, Microbiology & Immunology, Center for Oral Biology, University of Rochester Medical Center, Rochester, NY, 2008-2013
- Selected as Penn Fellows, University of Pennsylvania, 2016
- IADR/GlaxoSmithKline Innovation in Oral Care Award, 2013
- Honorary Professor, West China College of Stomatology, Sichuan University, China, 2011
- Best of USDA National Research Initiative Grants, 2009
- IADR Basil G. Bibby Young Investigator in Cariology Award, 2007IADR
- Distinguished Scientist Award – Young Investigator Award,
- 2006IADR/GlaxoSmithKline Innovation in Oral Care Award, 2006
- Excellence in Research Incentives Program – University of Rochester, 2006
- IADR/Colgate-Palmolive Research in Prevention Award, 2001
- The Basil G. Bibby Fellowship Award for Excellence in Oral Health Research, 2001
- Member, Hatton Awards Committee of the AADR, 2012-present
- Standing member, Special Grants Review Committee of the NIH/NIDCR, 2010-present
- Reviewer for NIH Study Sections & Special Emphasis Panels, 2008-present
- Editorial board of several journals, including Journal of Dental Research (2015-present), International Journal of Oral Science (2015-present), Frontiers in Cellular and Infection Microbiology (2012-present)