The Penn Dental Medicine Live Cell Imaging Core features a Nikon A1R Confocal Microscope, that includes the following specifications and applications.
- Transmitted light, DIC and epifluorescence (Dapi, FITC, TRITC filters)
- Objectives: 20x Dry, 40x Dry, 60x water, 100x oil
- Fully automated XYZ scanning stage — Piezo Z stage insert for high speed image capture when using resonance scanner; Perfect Focus System, allowing for accurate stage reposition over timed series events.
- Environmental chamber — Adjustable CO2 percentage and temperature with constant humidity; Accommodates 35 mm glass bottom culture dishes, 30×60mm chamber slides and 25×75mm glass slides.
- Scan Heads for Image capture — Galvo (up to 4 fps (512×512); Resonance 30 fps (512×512 up tp 230 fps (512×64)
- Stage — Fully automated XYZ scanning; Piezo Z stage insert for high speed image capture with resonance scanner; Perfect Focus System, allowing accurate stage reposition over a time series.
- 4 Laser lines with filter configuration providing the following:
|638||650 Long Pass|
- Detectors — 4- Dedicated PMT for laser lines; 1-DIC PMT; DUS Spectral detector- 32 channels of detection over the 400nm-750mn. Spectral resolution adjusted to 2.5nm, 6nm and 10nm channel width.
- Image Analysis — Nikon NIS Elements software; Additional Elements Software Workstation
- Examples of Applications — Live cell (GFP translocation, Phagocytic uptake, LPSinduced toxicity, Spectral profiling of inclusion bodies, Bimolecular fluorescence complementation) and Fixed (4-color imaging, Whole retina mounts, Protein co-localization, 3D targeted protein expression, FRET)
Penn Dental Medicine
240 S. 40th Street
514 Leon Levy Building
Dr. Anu Dhingra
513 Leon Levy Building
Dr. Kathleen Boesze-Battaglia
515 Leon Levy Building