Live Cell Imaging Core

The Penn Dental Medicine Live Cell Imaging Core features a Nikon A1R Confocal Microscope, that includes the following specifications and applications.

  • Transmitted light, DIC and epifluorescence (Dapi, FITC, TRITC filters)
  • Objectives: 20x Dry, 40x Dry, 60x water, 100x oil
  • Fully automated XYZ scanning stage — Piezo Z stage insert for high speed image capture when using resonance scanner; Perfect Focus System, allowing for accurate stage reposition over timed series events.
  • Environmental chamber — Adjustable CO2 percentage and temperature with constant humidity; Accommodates 35 mm glass bottom culture dishes, 30×60mm chamber slides and 25×75mm glass slides.
  • Scan Heads for Image capture — Galvo (up to 4 fps (512×512); Resonance 30 fps (512×512 up tp 230 fps (512×64)
  • Stage — Fully automated XYZ scanning; Piezo Z stage insert for high speed image capture with resonance scanner; Perfect Focus System, allowing accurate stage reposition over a time series.
  • 4 Laser lines with filter configuration providing the following:
Excitation Wavelengths
Emission Filters
405nm 425-475
457nm 465-505
488 500-550
514 525-565
561 575-625
638 650 Long Pass
  • Detectors — 4- Dedicated PMT for laser lines; 1-DIC PMT; DUS Spectral detector- 32 channels of detection over the 400nm-750mn. Spectral resolution adjusted to 2.5nm, 6nm and 10nm channel width.
  • Image Analysis — Nikon NIS Elements software; Additional Elements Software Workstation
  • Examples of Applications — Live cell (GFP translocation, Phagocytic uptake, LPSinduced toxicity, Spectral profiling of inclusion bodies, Bimolecular fluorescence complementation) and Fixed (4-color imaging, Whole retina mounts, Protein co-localization, 3D targeted protein expression, FRET)

Location

Penn Dental Medicine
240 S. 40th Street
514 Leon Levy Building

Contact

Dr. Anu Dhingra
dhingra@upenn.edu
513 Leon Levy Building
215-746-6022

Dr. Kathleen Boesze-Battaglia
battagli@upenn.edu
515 Leon Levy Building
215-898-9167

Reservation Email

dhingra@upenn.edu

Interested in attending Penn Dental Medicine? Admissions